Herein, we report a case of esophageal mucosa-associated lymphoid tissue lymphoma in a year-old man, which involved the use of a stacked forceps biopsy to obtain adequate samples for pathological analysis; the use of the stacked forceps biopsy method is unlike those used in previous cases.
The patient received cyclophosphamide, vincristine, and prednisolone chemotherapy; he achieved a complete response. In addition, we review the literature relevant to this case. Evaluation of adenoassociated virus 2 and human papilloma virus 16 and 18 infection in cervical cancer biopsies. Protective roles of adenoassociated virus AAV 2 in cervical tumorigenesis are controversial.
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In an effort to clarify this issue, we tested prevalence of AAV 2 and human papillomavirus HPV infection in cervical lesions and adjacent normal tissues. In 92 perilesional normal tissues , AAV 2 was detected in 53 cases Thus, these results support the notion that AAV 2 is not associated with cervical tumorigenesis. A year-old Hispanic male patient initially diagnosed with an EGFR 19 deletion positive lung adenocarcinoma and clinically responded to initial erlotinib treatment. Bioengineering a vaginal replacement using a small biopsy of autologous tissue.
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Many congenital and acquired diseases result in the absence of a normal vagina. Patients with these conditions often require reconstructive surgery to achieve satisfactory cosmesis and physiological function, and a variety of materials have been used as tissue sources. Currently employed graft materials such as collagen scaffolds and small intestine are not ideal in that they fail to mimic the physiology of normal vaginal tissue.
Engineering of true vaginal tissue from a small biopsy of autologous vagina should produce a superior graft material for vaginal reconstruction. This review describes our current experience with the engineering of such tissue and its use for vaginal reconstruction in animal models. Our successful construction and implantation of neovaginas through tissue engineering techniques demonstrates the feasibility of similar endeavors in human patients. Additionally, the use of pluripotent stem cells instead of autologous tissue could provide an "off-the-shelf" tissue source for vaginal reconstruction.
The role of skin and muscle biopsy in the diagnosis of main connective tissue diseases. Systemic involvement in autoimmune diseases is often unclear and organ changes are confounding, thus making it difficult to have an early accurate diagnosis. In those situations, both clinical and paraclinical findings might orientate the diagnosis, but only histological or immunohistochemistry changes might be accurate enough. The skin histological changes are relevant and sometimes might have a tremendous role in the accurate diagnosis of autoimmune rheumatic diseases, due to the correlation with the clinical systemic manifestations of the diseases and through the accessibility of biopsy.
In the same time, muscle biopsy can provide important support for physicians improving diagnosis and optimizing management of connective tissue diseases.
Use of a new jumbo forceps improves tissue acquisition of Barrett's esophagus surveillance biopsies. The major risk factors for the development of esophageal adenocarcinoma remain long-standing GERD and resultant Barrett's esophagus BE. Finding the exact method of adequate tissue sampling for surveillance of dysplasia in BE remains a dilemma.
We prospectively compared standard large-capacity biopsy forceps with a new jumbo biopsy forceps for dysplasia detection in BE. Prospective, single-center investigation. We prospectively enrolled 32 patients undergoing surveillance endoscopy for BE.
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Biopsy samples were obtained in paired fashion alternating between the experimental jumbo and control large-capacity forceps. Each sample was assessed for histopathology, specimen size, and adequacy. A total of specimens were available for analysis for this investigation. Six patients were found to have dysplasia, and in 5 of those patients, the dysplasia was only detected with the jumbo forceps.
The mean width was significantly greater in the Radial Jaw 4 jumbo group 3. The Radial Jaw 4 jumbo biopsy forceps significantly improves dysplasia detection and adequate tissue sampling in patients undergoing endoscopy for BE. Sentinel lymph node biopsy in early-stage cervical cancer: utility of intraoperative versus postoperative assessment. To determine the diagnostic accuracy of sentinel lymph node SLN detection using lymphoscintigraphy, intraoperative blue dye, and radiocolloid in patients with early-stage cervical cancer.
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Intra- cervical injection of technetium sulfur colloid and lymphoscintigraphy were performed preoperatively. Isosulfan blue was injected intra- cervically immediately prior to surgery. SLNs were excised and examined intraoperatively imprint cytology and frozen section and postoperatively H and E histology and immunohistochemistry IHC for cytokeratin. Thirty eight patients were evaluable. Laparoscopy and laparotomy were performed in However, while current intraoperative pathology techniques for assessing nodal metastases reliably detect metastases larger than 2 mm, they lack sufficient sensitivity to detect micrometastasis and isolated tumor cells.
Comparison of different biopsy forceps models for tissue sampling in eosinophilic esophagitis. Background and aims: Eosinophilic esophagitis EoE is a mixed inflammatory and fibrostenotic disease. Unlike superficial inflammatory changes, subepithelial fibrosis is not routinely sampled in esophageal biopsies. This study aimed to evaluate the efficacy and safety of deep esophageal sampling with four different types of biopsy forceps.
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Patients and methods: In this cross-sectional study, esophageal biopsies were taken in 30 adult patients by one expert endoscopist. Use of hydrogel breast biopsy tissue markers reduces the need for wire localization. The purpose of this study was to determine whether breast biopsy tissue markers composed of an ultrasound-visible hydrogel reduced the need for preoperative wire localization WL in patients undergoing a partial mastectomy.
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A single-surgeon, single-institution, retrospective chart review was performed on consecutive female patients, with mean age 67 years range years , from to undergoing partial mastectomies after percutaneous biopsies by stereotactic or ultrasound guidance.
Overall, the use of WL was more frequent in patients who had standard other markers placed during biopsy as opposed to those with hydrogel markers HydroMARK. For stereotactic biopsy , HydroMARK was as effective as a standard marker in terms of partial mastectomy specimen volume and re-excision rate. This yields potential for cost savings, increased efficacy in operating room and radiology scheduling, and patient comfort and convenience.
Needle endomicroscope with a plastic, achromatic objective to perform optical biopsies of breast tissue. In order to diagnose cancer in breast tissue , a sample must be removed, prepared, and examined under a microscope. To provide an alternative to conventional biopsies , an endomicroscope intended to perform optical biopsies is demonstrated. The system provides high resolution, high contrast images in real-time which could allow a diagnosis to be made during surgery without the need for tissue removal.
Optical sectioning is achieved via structured illumination to reject out of focus light. An image is relayed between the sample plane and the imaging system by a coherent fiber bundle with an achromatized objective lens at the distal tip of the fiber bundle which is the diameter of a biopsy needle. The custom, plastic objective provides correction for both the excitation and emission wavelengths of proflavine nm and nm, respectively.
It also magnifies the object onto the distal tip of the fiber bundle to increase lateral resolution. The lenses are fabricated via single point diamond turning and assembled using a zero alignment technique. The lateral resolution and chromatic focal shift were measured and in vitro images of breast carcinoma cells stained with proflavine were captured. The optical biopsy system is able to achieve optical sectioning and to resolve smaller features than the current high resolution microendoscope.
Cryotherapy for HPV clearance in women with biopsy -confirmed cervical low-grade squamous intraepithelial lesions. To compare the clearance rate of HPV infection among women aged older than 30 years with biopsy -confirmed cervical low-grade squamous intraepithelial lesions LSIL 1 year after cryotherapy with the spontaneous clearance rate observation.
HPV DNA typing by polymerase chain reaction and reverse line blot hybridization were used to identify 14 high-risk types and 23 low-risk types. Between December and March , women were recruited to the study and 60 cases had positive results on HPV testing.
Dissertations & Theses from 12222
Twenty-nine patients were randomly allocated to the cryotherapy group and 31 to the observation group. At 1 year, However, in coupling with visual inspection with acetic acid as a single visit approach, its effect on prevention of HSIL and cervical cancer is still promising.
Therefore, cryotherapy should not be withdrawn from such programs. Copyright Elsevier Ireland Ltd. A definitive diagnosis of mucosa-associated lymphoid tissue lymphoma made at a second biopsy. A case of mucosa-associated lymphoid tissue MALT lymphoma, the most frequent of the various conjunctival lymphoproliferative disorders, in which the initial biopsy was inconclusive but the second biopsy provided a definitive diagnosis, is reported.
A year-old woman with a 3-month history of bilateral conjunctival swelling was referred by a local physician for suspected MALT lymphoma. A salmon-pink elastic swelling was found to involve both eyes and to extend from the lower palpebral conjunctiva to the bulbar conjunctiva.